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Cytotoxic T‐lymphocyte escape viral variants: how important are they in viral evasion of immune clearance in vivo?
Summary: Although viral variants which are not recognized by epitope‐specific cytotoxic T lymphocytes (CTL) have been shown lo arise during a number of persistent virus infections, in many cases their significance remains controversial: it has been argued that the immune response is sufficiently plastic to contain thei...
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Fungal Speciation Using Quantitative Polymerase Chain Reaction (QPCR) in Patients With and Without Chronic Rhinosinusitis
Objectives/Hypothesis: The objectives of this study were to determine the mycology of the middle meatus using an endoscopically guided brush sampling technique and polymerase chain reaction laboratory processing of nasal mucous; to compare the mycology of the middle meatus in patients with sinus disease with subjects w...
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Role of aminopeptidase N (CD13) in tumor‐cell invasion and extracellular matrix degradation
We have investigated the effect of monoclonal antibodies (MAbs) specific for aminopeptidase N/CD13 on the invasion of human metastatic tumor cells into reconstituted basement membrane (Matrigel). The invasion of human metastatic tumor cells (SN12M renal‐cell carcinoma, HT1080 fibrosarcoma and A375M melanoma) into Matri...
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Virulent and attenuated canine distemper virus infects multiple dog brain cell types in vitro
Canine Distemper Virus (CDV) produces an encephalitis in dogs that varies with viral strain. We have studied the cell tropisms of two virulent strains (CDV‐SH and CDV A75–17) and an attenuated strain, Rockborn (CDV‐RO), in cultured canine brain cells. Infected cell types were identified by double immunofluorescent labe...
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Mortality factors affecting the leaf‐mining stages of Phyllonorycter (Lepidoptera: Gracillariidae) on oak and birch: 2. Biology of the parasite species
Parasitic Hpenoptera attacking Phyllonorycter species mining leaves of oak and birch were studied at a Cheshire locality during 1974. The host developmental stages killed, and attacked, by each parasite species are analysed. Smaller species and males tend to kill earlier Phyllonorycter instars than do larger species an...
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Mycobacteria and the Skin
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Prevalence and Risk Factors for Bacterial Food‐Borne Zoonotic Hazards in Slaughter Pigs: A Review
The Hygiene Package and Regulation EC‐2160/2003 require information flow from farm to slaughterhouse to enhance European consumers protection in a ‘farm to fork’ approach. This obligation especially concerns food‐borne zoonotic hazards transmitted to humans through pork consumption, such as thermophilic Campylobacter s...
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Coronavirus y radiología. Consideraciones sobre la crisis
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Scalp Verruca from Barber Clippers: an Epidemiologic Assessment of a Common Fomite
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Inactivation of interleukin‐8 by aminopeptidase N (CD13)
Aminopeptidase (APN) was found to degrade interleukin‐8 (IL‐8) and inactivate its chemotactic activity. The chemotactic activity of IL‐8 was decreased by APN or neutrophil plasma membranes dose‐ and time‐dependently. The chemotactic activity was not inactivated in the presence of bestatin or WM15 monoclonal antibody. T...
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Rat tracheal organ culture supports replication of parainfluenza 1 (6/94) virus and promotes 6/94 virus rescue from latently infected human brain cells
Rat tracheal organ culture (TOC) supported replication of parainfluenza 1 (6/94) virus. Cell‐associated and cell‐free viruses were found after primary infection of TOC. In contrast to other mammalian systems, rat TOC was capable of maintaining 6/94 virus infectivity after primary infection. Rat TOC may be considered a ...
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Acute Respiratory Tract Infection in Daycare Centers for Older Persons
OBJECTIVE: To evaluate the rate of specific pathogens and clinical syndromes associated with acute respiratory tract infections (ARTI) in frail older persons attending daycare. DESIGN: Prospective descriptive study, without intervention. SETTING: Two sites of a senior daycare program providing all‐inclusive care for th...
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Evaluation of a new enzyme‐linked immunosorbent assay (ELISA) in the diagnosis of rhinovirus infection
This study describes the evaluation of a newly developed ELISA for the direct detection of rhinovirus antigens in nasal washings. Of 54 volunteers inoculated with 100 TCID(50) of human rhinovirus type 2 (HRV‐2), 50 (96.6%) and 32 (59%) excreted antigen and virus on at least 1 of 3 days investigated, respectively. Thirt...
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Morphometric patterns in Recent and fossil penguins (Aves, Sphenisciformes)
A total of 622 skin specimens, 527 skeletons, and myological data compiled by Schreiweis (1972) were used to investigate morphometric patterns within and among the 18 Recent species of Spheniscidae, and to compare the family with a fighted species, the common diving‐petrel (Pelecanoides urinator), considered by some au...
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Introduction: why don't HIV/AIDS policies work?
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21st Annual Symposium 25–27 September 2008 Rhodes, Greece Programme of the 21st annual meeting on: Peripheral Nerve, Neuromuscular and Muscle Disease
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Studies of an outbreak of acute hepatitis A: II. Antibody changes to cytomegalovirus and herpesvirus
The acute and convalescent sera from 14 schoolchildren with acute hepatitis A were tested for antibody changes to 70 viral antigens. Marked decreases were noted in the levels of antibody to cytomegalovirus in 5 of the 14 children and in the levels of antibody to herpesvirus type 1 in 3. No such changes were noted in 9 ...
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European College of Veterinary Internal Medicine‐Companion Animals
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Two Nursing Home Outbreaks of Respiratory Infection with Legionella sainthelensi
OBJECTIVE: To describe outbreaks of infection caused by Legionella sainthelensi occurring in older residents of two nursing homes and to determine risk factors for the development of infection. DESIGN: Descriptive epidemiology and a case‐control study. SETTING: Two nursing homes (140 beds and 254 beds in nursing homes ...
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Localization of rhinovirus replication in vitro with in situ hybridization
To facilitate understanding of human rhinovirus (HRV) pathogenesis, methods were developed for detection of HRV infection in vitro using in situ hybridization (ISH). HRV‐14 RNA probes and oligonucleotide probes representing conserved sequences in the 5′‐non‐translated region were labeled with (35)S and used to detect i...
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Role of the golgi apparatus in cellular pathology
The Golgi apparatus response to pathological disorders is predominantly as an intermediary component of membrane biogenesis where it is involved in processing, sorting and secretion of materials via secretory granules, and in the formation of lysosomes. A common initial response of the Golgi apparatus to any stress is ...
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CHILDHOOD ASTHMA IN BRISBANE: EPIDEMIOLOGICAL OBSERVATIONS
Analysis of hospital admissions for 20 years suggests that there has been an increase in childhood asthma in Brisbane. The characteristic seasonal pattern of asthma with waves in autumn and spring is evident from the second year of age and continues into adult life. It has not been explained, although respiratory infec...
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IDENTIFICATION OF SEEDS FROM VARIOUS SPECIES OF STROPHANTHUS
In a search for differential specific characters, the outer epidermis of the testa from Strophanthus seeds of 15 species has been examined. A “trichome index” has been defined, and the method of its determination described. It enabled the species studied to be divided into six groups.
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A novel method for determination of inorganic oxyanions by electrospray ionization mass spectrometry using dehydration reactions
Novel methods for the determination of inorganic oxyanions by electrospray (ES) ionization mass spectrometry have been developed using dehydration reactions between oxyanions and carboxylic acids at the ES interface. Twelve oxyanions (VO(3) (−), CrO(4) (2−), MoO(4) (2−), WO(4) (2−), BO(3) (3−), SiO(3) (2−), SiO(4) (4−)...
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Viral Respiratory Infections in the Institutionalized Elderly: Clinical and Epidemiologic Findings
OBJECTIVE: To prospectively evaluate the incidence and impact of viral respiratory infection in the institutionalized elderly during a winter season. DESIGN: Prospective descriptive study, without intervention. METHOD: Patients with respiratory illnesses were evaluated by a directed history and physical examination. Na...
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A novel real‐time PCR system for simultaneous detection of human viruses in clinical samples from patients with uncertain diagnoses
A novel simultaneous detection system for human viruses was developed using a real‐time polymerase chain reaction (PCR) system to identify causes of infection in clinical samples from patients with uncertain diagnoses. This system, designated as the “multivirus real‐time PCR,” has the potential to detect 163 human viru...
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Nitrogen fixing bacteria in the family Acetobacteraceae and their role in agriculture
For centuries, the Acetobacteraceae is known as a family that harbors many species of organisms of biotechnological importance for industry. Nonetheless, since 1988 representatives of this family have also been described as nitrogen fixing bacteria able to plant growth promotion by a variety of mechanisms. Nitrogen fix...
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Development and evaluation of a real‐time nucleic acid sequence based amplification assay for rapid detection of influenza A
The development and introduction of effective treatment for influenza A in the form of neuraminidase inhibitors have made the rapid diagnosis of infection important especially in high‐risk populations. The aim of this study was to develop a real‐time nucleic acid sequenced based amplification (NASBA) using a molecular ...
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Oxidation of N‐heterocyclics: A green approach
[Chemical structure: see text] Environmentally benign oxidation methods satisfy the postulates of green chemistry. Heterocyclic Noxides have applications in synthetic organic chemistry, chemotherapy and agrochemicals. Synthesis of Noxides using green oxidants will be attractive over the conventional methods. The presen...
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First detection of spring viraemia of carp virus in common carp (Cyprinus carpio L.) affected by a septicaemic disease in Mexico
Spring viraemia of carp (SVC) is an infectious disease responsible for severe economic losses for various cyprinid species, particularly common carp (Cyprinus carpio carpio). The causative agent is the SVC virus (SVCV), a member of the Sprivivirus genus, Rhabdoviridae family, and a List 1 pathogen notifiable by the Wor...
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The role of the combination of IL‐2 and TGF‐β or IL‐10 in the generation and function of CD4(+) CD25(+) and CD8(+)regulatory T cell subsets
Recently, considerable attention has been focused on thymus‐derived CD4(+) regulatory T cells that constitutively express CD25 and have a contact‐dependent, cytokine‐independent mechanism in vitro. However, peripheral CD4(+) and CD8(+) T cells can also be induced to become regulatory T cells. Here we review our studies...
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Diseases of pet rodents
The main pet rodent species are introduced with some brief notes on their suitability as pets and normal behaviour. The general clinical features of disease are discussed with some emphasis on species variation. The important diseases that occur in the United Kingdom are described under the headings of respiratory dise...
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Tolerance of one‐month intranasal interferon
Under double‐blind conditions, groups of volunteers (68 in total) were allocated at random to take intranasal solutions of placebo or one of three doses of highly purified leucocyte interferon by intranasal spray twice a day for 28 days. The highest dose would have been expected to protect against experimental colds. T...
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Detection and identification of viruses by electron microscopy
Electron microscopy can aid in the rapid diagnosis of viral diseases, as it can be performed in a matter of hours, but on a routine basis it should be used in conjunction with other techniques. Initially, the specimen source and patient symptoms should be ascertained, as these will lend suggestions of possible agents w...
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SHELF‐LIFE EXTENSION OF FRESH FISH — A REVIEW PART I — SPOILAGE OF FISH
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Giardia lamblia in children and the child care setting: A review of the literature
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Ecto‐enzymes: physiology meets pathology
Ecto‐enzymes are catalytic membrane proteins with their active sites outside the cell. They include cholinesterase, which inactivates acetylcholine, and angiotensin‐converting enzyme, which converts angiotensin I to biologically active angiotensin II, and numerous other peptidases, transpeptidases, nucleotidases, phosp...
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Inhibition of Phagocytosis and Interleukin‐1 Production in Pulmonary Macrophages From Rats With Sialodacryoadenitis Virus Infection
To test whether or not sialodacryoadenitis virus (SDAV) infection in rats affects pulmonary macrophage function, we intranasally inoculated pathogen‐free F344 rats with SDAV and collected alveolar and interstitial macrophages 5 d later. We assessed Fc receptor‐mediated attachment and phagocytosis by phase‐contrast micr...
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Body elongation and decreased reproductive output within a restricted clade of lizards (Reptilia: Scincidae)
Relationships between body shape and relative abdominal size were compared among differentially elongate species within the scincid lizard genus Brachymeles, to investigate how morphological evolution affects the proportion of body volume available to hold eggs and offspring. Relative abdominal size is inversely relate...
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Non‐Influenza Respiratory Viruses May Overlap and Obscure Influenza Activity
OBJECTIVE: To report the number and timing of influenza A isolates, as well as overlapping respiratory viruses. Co‐circulating respiratory viruses may obscure the determination of influenza activity. DESIGN: Prospective clinical surveillance for the new onset of respiratory illness followed by viral cultures during sev...
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Astrocytic reaction predominance in chronic encephalitis of junin virus‐infected rats
Junin virus antigen distribution and astrocytic reaction to prolonged infection were characterized in rat brain by the PAP technique. During the acute stage of neurologic disease following intracerebral inoculation, Junin antigen was detected in 100% of animals, strongly in most neurons but also to a much lesser degree...
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Evaluation of an enzyme‐linked immunosorbent assay that measures rhinovirus‐specific antibodies in human sera and nasal secretions
Rhinovirus‐specific antibodies have traditionally been detected by their ability to neutralise the homologous rhinovirus serotype in tissue culture. Recently, however, we have described an enzyme‐linked immunosorbent assay that detects rhinovirus‐specific antibodies in sera and nasal secretions [Barclay and Al‐Nakib, 1...
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Microstructure, evolution, and ontogeny of scale surfaces in cordylid and gerrhosaurid lizards
The distal oberhautchen and proximal beta layer of 41 species and every genus of cordylid and gerrhosaurid lizard were examined with a scanning electron microscope. Scale organs in these families are concentrated on the face, and scale organs occur singly or in pairs below the keel on the dorsals of cordylids but not g...
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IN VITRO EFFECTS OF MONOLAURIN COMPOUNDS ON ENVELOPED RNA AND DNA VIRUSES
Monolaurin alone and monolaurin with tert‐butylhydroxyanisole (BHA), methylparaben, or sorbic acid were tested for in vitro virucidal activity against 14 human RNA and DNA enveloped viruses in cell culture. At concentrations of 1% additive in the reaction mixture for 1 h at 23°C, all viruses were reduced in infectivity...
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Evolution of the middle ear apparatus in talpid moles
The middle ear structures of eight species of mole in the family Talpidae (Mammalia: Eulipotyphla) were studied under light and electron microscopy. Neurotrichus, Parascalops, and Condylura have a simple middle ear cavity with a loose ectotympanic bone, ossicles of a “microtype” morphology, and they retain a small tens...
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Interactions of Pseudorabies Virus With Swine Alveolar Macrophages: Effects of Virus Infection on Cell Functions
In order to assess the effect of Pseudorabies virus (PRV) infection on the function of swine alveolar macrophages (AM), lung lavage cells were cultured, infected with one of six strains of PRV, and various activities were measured. Activity measurement included viability, phagocytosis of yeast, phagosome‐lysosome fusio...
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Appearance of complement components and immunoglobulins on nasopharyngeal epithelial cells following naturally acquired infection with respiratory syncytial virus
Nasopharyngeal epithelial cells (NPEC) were collected from 144 infants and children with respiratory syncytial virus (RSV) infection, and were analyzed by fluorescent antibody techniques for the presence of cell‐bound complement (C(/) (3)), IgA, IgC, and IgM class of immunoglobulins (Ig), and respiratory syncytial viru...
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SCIENTIFIC INFORMATION DOCUMENT: Campylobacter infection
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Innovative Drug Treatments for Viral and Autoimmune Myocarditis
Myocarditis has been shown to be a common cause of cardiomyopathy and is believed to account for 25% of all cases in human beings. Unfortunately, the disease is difficult to detect before a myopathic process ensues. Treatment of myocarditis‐induced heart failure includes the standard regimen of diuretics, digoxin, angi...
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Program and Abstracts of the 11th INTERNATIONAL RES CONGRESS and 24th NATIONAL MEETING OF THE RETICULOENDOTHELIAL SOCIETY: Westin Kauai, Kauai, Hawaii, October 17–21, 1987
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Enzyme immunoassay for respiratory syncytial virus: Rapid detection in nasopharyngeal secretions and evaluation of isolates representing different RSV subgroups
The presence of respiratory syncytial virus (RSV) was investigated by immunofluorescent antibody (IFA) technique and by an enzyme immunoassay (EIA) in 169 samples of nasopharyngeal secretions of infants and children with acute respiratory infections. Of 31 samples positive by EIA, 25 were positive by IFA. In 24 samples...
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defensive publications in an r&d race
This paper considers a multistage dynamic R&D race in which the competitors strategically publish research findings. Publications change the prior art, thus affecting patentability. Firms publish when they are behind in the race and their rival is close to winning it. Publication sets back both competitors and gives th...
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Molecular biology of human pathogenic viruses
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Refining rare weed trait syndromes along arable intensification gradients
QUESTION: How does the conservation (rarity) value of arable weed communities differ along intensification gradients? Which functional traits best distinguish the weed communities of more and less extensively managed fields? Can the same traits predict the rarity of individual weed species? LOCATION: Hungary. METHODS: ...
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The effect of plant species on soil nitrogen mineralization
1.. To ascertain the influence of different plant species on nitrogen (N) cycling, we performed a long‐term garden experiment with six grasses and five dicots with different potential growth rates, that are adapted to habitats with different nutrient supplies. We measured in situ N mineralization and nitrification of t...
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Current issues and forthcoming events
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Enzyme‐Linked lmmunosorbent assay: A rapid reproducible test for the measurement of rabies antibody
An enzyme‐linked immunosorbent assay (ELISA) was developed for detecting IgG antibodies to the Pitman Moore strain of rabies virus in sera from subjects immunised with HDCS vaccine. End‐point titres of antibody were determined using a pocket calculator preprogrammed to analyse absorbence values of test sera and negativ...
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Polymerase chain reaction and sequencing for typing rhinovirus RNA
Primers were designed and tested for their ability to distinguish rhinoviruses from enterovi‐ruses. A primer set derived from the 5′‐UTR/VP coding region junction was able to amplify all the rhinovirus serotypes tested. Enteroviruses were either not amplified by these primer pairs or produced a band of larger size that...
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Detection of enteric adenoviruses with synthetic oligonucleotide probes
The abilities of hybridization probes to detect all human adenovirus types and to identify enteric adenovirus types were evaluated. The efficiency of hybridization was compared to other tests currently in routine laboratory use on clinical specimens from young children with gastroenteritis, Probes were derived from var...
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Mitotane (o, p‘‐DDD) treatment in a cat with hyperadrenocorticism
An 11‐year‐old male castrated Persian cat with spontaneous hyperadrenocorticism was presented. Both adrenals were grossly enlarged and calcified. A diagnosis of pituitary‐dependent hyperadrenocorticism was made. Signs of hyperadrenocorticism resolved with long‐term mitotane treatment. Concurrent diabetes mellitus resol...
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Detection of cytomegalovirus and Epstein‐Barr virus in labial salivary glands in Sjogren's syndrome and non‐specific sialadenitis
To investigate the role of herpes viruses in Sjogren's syndrome, minor (labial) salivary gland tissues from Sjogren's syndrome and from non‐specific sialadenitis were examined for Epstein‐Barr virus (EBV) and human cytomegalovirus (HCMV) DNA by the polymerase chain reaction. Almost half of all salivary glands studied c...
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Prospective Hematologic and Clinicopathologic Study of Asymptomatic Cats With Naturally Acquired Feline Immunodeficiency Virus Infection
Prospective studies were performed over a 28‐ to 77‐month period (median, 66 months) on 5 cats with naturally acquired feline immunodeficiency virus (FIV) infection in an attempt to correlate hematologic and Clinicopathologic changes with the emergence of clinical disease. On presentation, all cats were asymptomatic; f...
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The accuracy of nurses' estimates of their absenteeism
gaudine a & gregory c (2010) Journal of Nursing Management 18, 599–605 The accuracy of nurses' estimates of their absenteeism Aim The purpose of the present study was to determine the accuracy of nurses’ self‐reports of absence by examining: (1) the correlation, intra‐class correlation, and Cronbach’s alpha for self‐re...
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Characterization of precipitates size distribution: validation of low‐voltage STEM
The size distribution of second phase precipitates is frequently determined using conventional transmission electron microscopy (CTEM). However, other techniques, which present different advantages, can also be used for this purpose. In this paper, we focus on high angle annular dark field (HAADF) in TEM and scanning T...
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Can plant litter affect net primary production of a typical steppe in Inner Mongolia?
Question: Litter (dead leaves or stems) affects production by conserving soil moisture. However, that role is not clear for grasslands where most precipitation falls during the growing season when the demand for water is high. Our question was: Does litter affect forage production in such an environment? Location: Typi...
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Functional traits explain amphibian distribution in the Brazilian Atlantic Forest
AIM: Species distributions are one of the most important ways to understand how communities interact through macroecological relationships. The functional abilities of a species, such as its plasticity in various environments, can determine its distribution, species richness and beta diversity patterns. In this study, ...
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Probable Vitamin K‐Deficient Bleeding in Two Cats With Malabsorption Syndrome Secondary to Lymphocytic Plasmacytic Enteritis
Two cats with intestinal malabsorption developed a hemorrhagic diathesis. Although unsubstantiated, the probable cause of bleeding was a chronic malabsorption of fat and the fat‐soluble vitamin K. When treated_with vitamin Ki per os, one cat's clotting times were only partially corrected. Since vitamin K, is actively a...
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Ultrastructure of Euspermatozoa of cerithiacean gastropods (Prosobranchia: Mesogastropoda)
Euspermatozoa of selected cerithiacean gastropods have been studied using transmission electron microscopy and the results compared (primarily) with previous studies of mesogastropod and neogastropod euspermatozoa. Cerithiacean euspermatozoa each possess a well‐defined acrosome (extremely varied in shape), a short (2.2...
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Gastrointestinal Motility and Disease in Large Animals
An understanding of the relationship between gastrointestinal (GI) motility and disease is imperative for the proper treatment of large animal patients, especially as new therapeutic agents become available. However, the abundance of information that has become available in the last 2 decades makes gaining this underst...
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Lymphocyte activation
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Aetiology and outcome in 90 cats presenting with dyspnoea in a referral population
Objectives: Dyspnoea is an unspecific severe presenting sign, which can be life threatening and requires prompt treatment. Dyspnoeic cats often have significant underlying disease. Underlying aetiologies in cats that presented with dyspnoea were reviewed, and associations with patient signalment and outcome were invest...
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Minireview The influence of season, photoperiod, and pineal melatonin on immune function
Abstract: In addition to the well‐documented seasonal cycles of mating and birth, there are also significant seasonal cycles of illness and death among many animal populations. Challenging winter conditions (i.e., low ambient temperature and decreased food availability) can directly induce death via hypothermia, starva...
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A New Disease Emerges: Severe Acute Respiratory Syndrome (SARS)
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Effect of Gd‐DTPA‐BMA on blood and myocardial T(1) at 1.5T and 3T in humans
PURPOSE: To compare T(1) values of blood and myocardium at 1.5T and 3T before and after administration of Gd‐DTPA‐BMA in normal volunteers, and to evaluate the distribution of contrast media between myocardium and blood during steady state. MATERIALS AND METHODS: Ten normal subjects were imaged with either 0.1 mmol/kg ...
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Meeting report on the 12th International Congress of Histochemistry and Cytochemistry (ICHC)
The International Congress of Histochemistry and Cytochemistry (ICHC) promoted in San Diego La Jolla (CA, USA), the 12th meeting where researchers of all over the world presented their work and the most innovative methods in histochemical disciplines. A summary of the last meeting is reported. © 2005 Wiley‐Liss, Inc.
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Chikungunya disease and chloroquine treatment
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Diversity and distribution of ferns in sub‐Saharan Africa, Madagascar and some islands of the South Atlantic
Aim This paper reports the diversity and endemism patterns of African ferns, and explores the potential role of diversity refuges and environmental and historical factors in the shaping of these patterns. Material and locations The extant fern taxa occupying Africa south of the Sahara, Madagascar and some islands of th...
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Plasmapheresis combined with interferon: An effective therapy for multiple sclerosis
The rationale for the use of interferon (IFN) in the treatment of multiple sclerosis (MS) is based on its recognized antiviral and immunomodulating actions. The pathogenesis of MS is believed to be due to an immunologic response in a genetically predisposed individual, localized within the central nervous system white ...
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Rhinovirus C is associated with wheezing and rhinovirus A is associated with pneumonia in hospitalized children in Morocco
Human rhinovirus (RV) is commonly associated with severe acute lower respiratory infections (ALRI) in children. We aimed to describe the distribution of RV species and associations between RV species and clinical features in children hospitalized with clinically severe pneumonia (CSP) in Morocco. Nasopharyngeal aspirat...
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Protective effect of OK‐432 (streptococcal preparation) on murine fulminant hepatitis following mouse hepatitis virus infection
The effects of OK‐432 (streptococcal preparation) on murine fulminant hepatitis were investigated. Hepatitis was induced by injection of mouse hepatitis virus type 2 (MHV‐2) at a strength of either 1 times 10(3) or 1 times 10(4) plaque‐forming units (PFU). Mice without OK‐432 treatment died within 5 days, whereas mice ...
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WSAVA Guidelines for the vaccination of dogs and cats
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Expression and function of chemokines during viral infections: from molecular mechanisms to in vivo function
Recruitment and activation of leukocytes are important for elimination of microbes, including viruses, from infected areas. Chemokines constitute a group of bioactive peptides that regulate leukocyte migration and also contribute to activation of these cells. Chemokines are essential mediators of inflammation and impor...
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Clinical Practice Guideline for the Evaluation of Fever and Infection in Older Adult Residents of Long‐Term Care Facilities: 2008 Update by the Infectious Diseases Society of America
Residents of long‐term care facilities (LTCFs) are at great risk for infection. Most residents are older and have multiple comorbidities that complicate recognition of infection; for example, typically defined fever is absent in more than one‐half of LTCF residents with serious infection. Furthermore, LTCFs often do no...
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Systemic Vasculitis with Severe Cutaneous Manifestation as a Suspected Idiosyncratic Hypersensitivity Reaction to Fenbendazole in a Cat
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BSAVA NEWS
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Feline Hybridoma Growth Factor/lnterleukin‐6 Activity
An assay system was developed to measure feline hybridoma growth factor (HGF)/interleukin‐6 (IL‐6) activity in biological samples containing many kinds of cytokines by using the proliferation of the newly established mouse‐rat hybridoma clone. B3B1. The proliferative response of this B3B1 clone was IL‐6‐specific, and c...
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Direct detection of rhinoviruses by an enzyme‐linked immunosorbent assay
This paper describes the first enzyme‐linked immunosorbent assay for the detection of rhinovirus antigens in clinical specimens (nasal washings), either directly or following overnight cell culture amplification. The assay takes approximately 48 hours to perform and utilizes the same rabbit antirhinovirus hyperimmune s...
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Preliminary Characterisation of Torovirus‐Like Particles of Humans: Comparison With Berne Virus of Horses and Breda Virus of Calves
Pleomorphic virus‐like particles have been observed by electron microscopy in the faeces of children and adults with diarrhoea. Some of these particles were approximately 100 nm in diameter and had a “fringe” of closely applied peplomers approximately 10 nm long; they closely resembled Berne virus of horses and Breda v...
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Two models of multiple sclerosis: Experimental allergic encephalomyelitis (EAE) and theiler's murine encephalomyelitis virus (TMEV) infection. A pathological and immunological comparison
Theiler's murine encephalomyelitis virus (TMEV) infection and experimental allergic encephalomyelitis (EAE) are considered among the best models of human multiple sclerosis (MS). In both models, clinical disease is characterized by paralysis, while pathological changes consist of inflammatory demyelination. In both mod...
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Genetic variability of alcohol dehydrogenase among Australian Drosophila species: Correlation of ADH biochemical phenotype with ethanol resource utilization
Alcohol dehydrogenase (ADH) activities, electrophoretic phenotypes, and the extent of ethanol resource utilization are compared for three groups of species distinguishable on ecological criteria: 1) the cosmopolitan species D. melanogaster, a frequent inhabitant of wineries; 2) fruit‐baited species of the typically Aus...
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High protection of animals lethally infected with influenza virus by aprotinin‐rimantadine combination
The successful therapeutic synergism of aprotinin and rimantadine, which are known to attack different viral targets, was demonstrated in influenza‐virus infected animals. Combined treatment with these drugs of mice infected with a highly lethal does of mouse‐adapted influenza virus prevented the development of separat...
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WSAVA Guidelines for the Vaccination of Dogs and Cats
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BSAVA Companion
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Therapeutic spectrum of interferon‐β in ischemic stroke
Ischemic stroke is devastating and a major cause of morbidity and mortality worldwide. To date, only clot retrieval devices and/or intravenous tissue plasminogen activators (tPA) have been approved by the US‐FDA for the treatment of acute ischemic stroke. Therefore, there is an urgent need to develop an effective treat...
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RSV‐hRV co‐infection is a risk factor for recurrent bronchial obstruction and early sensitization 3 years after bronchiolitis
To assess risk factors of recurrent bronchial obstruction and allergic sensitization 3 years after an episode of acute bronchiolitis, whether after ambulatory care treatment or hospitalization. A monocentric prospective longitudinal study including infants aged under 1 year with acute bronchiolitis was performed, with ...
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Toxicologic effects of ribavirin in cats
Ribavirin, a broad‐spectrum antiviral agent active in vitro against a number of RNA and DNA viruses, has been associated with moderate toxicity in laboratory animals and humans. Clinically, ribavirin has been used effectively in persons primarily to treat life‐threatening viral diseases such as acute haemorrhagic fever...
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Occurrence in water of viruses of public health significance
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Detection of human bocavirus in respiratory, fecal, and blood samples by real‐time PCR
Human bocavirus (HBoV) has been detected worldwide in respiratory samples. Two real‐time PCR assays, targeting the non‐structural protein (NP‐1) and viral protein (VP‐1) genes, were designed and validated to detect HBoV in patients with respiratory disease, gastroenteritis, or systemic illness. Sensitivity of the NP‐1 ...
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ORIGINAL ARTICLE: Taxonomy and diversity of Afroalpine Chironomidae (Insecta: Diptera) on Mount Kenya and the Rwenzori Mountains, East Africa
Aim Anthropogenic climate change is expected to result in the complete loss of glaciers from the high mountains of tropical Africa, with profound impacts on the hydrology and ecology of unique tropical cold‐water lakes located downstream from them. This study examines the biodiversity of Chironomidae (Insecta: Diptera)...
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Isolation and Identification of Enteric Adenoviruses
Thirty‐four out of 64 faecal samples with adenovirus particles, as seen by electron microscopy, were found to contain adenovirus 40 or 41 by direct isolation and neutralization in Chang's conjunctival cells, mostly within one week. (Ad40 and 41 candidate viruses are serologically related.) 6 other adenovirus specics we...
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